Cytotoxicity study of AH26 and Amalgam, in vitro, using

Human Periodontal Ligament Fibroblasts 

S. Al-Nazhan, BDS, MDS*, L. Spangberg, DDS**, PhD
* Department of Restorative Dental Sciences, King Saud University College of Dentistry, P.O. Box 60169, Riyadh 11545, Saudi Arabia
**Department of Restorative Dentistry and Endodontology, School of Dental Medicine, University of Connecticut Health Center, Farmington, Connecticut, USA

The toxicity of two endodontic filling materials (AH 26 and amalgam) were evaluated in vitro using the radiochromium release method and primary culture of human periodontal ligament (PDL) fibroblasts. The materials were tested fresh and when set after one day. Results showed a high 51Cr - release when the materials were tested fresh. The level of the ''Cr-release decreased when the materials were allowed to set for one day. The results indicated that the human PDL fibroblast is an appropriate cell to use when evaluating the cytotoxicity of endodontic filling materials, in vitro, since they are in close contact with the tissue of periodontal ligament in case of clinical situations.

Saudi Dental Journal 1990;2(2):48-51.