06. Clinical  application of  guided   tissue  regeneration observation in saudi treated cases.

Mohamed Nassar,
Hera General Hospital, Makkah, Saudi Arabia.

Guided tissue regeneration techniques, using various membranes have become an exciting addition to therapeutic periodontal armamentarium. The lecture will discuss the principles, and the application of guided tissue regeneration with expanded polytetrafluroethylene in clinical and animal studies. It will cover the procedural guidelines for case selection, surgical technique follow-up cases, recent advances that enabled scientists to understand biological principles of wound healing.

The lecture will focus on application of this technique in Saudi Arabia treated cases.
Saudi Dental Journal 1992;4(SI)-Abstr.06:p7.

07.    "Goretex" guided tissue regeneration.

Regeneration has been defined as the biological process by which the architecture and function of the lost tissue is completely renewed.

For periodontally-involved teeth, this would mean formation of new cementum, periodontal ligament, and bone supporting previously diseased roots.

The Goretex periodontal membrane is used in guiding tissue regeneration in surgical periodontal therapy. It excludes gingival connective tissue and epithelium from the periodontal defect space, allowing periodontal ligament and alveolar bone cells to fill this space.

This lecture will discuss the use of the material to treat periodontal bony defects in humans.
Saudi Dental Journal 1992;4(SI)-Abstr.07:p8.

One of the most serious injuries which the dentist and the oral surgeon called upon to diagnose and treat are fractures of mandible, maxilla, and facial skeleton.

These cases are by no means uncommon and are caused principally by traffic accidents, motorcyclists and motorists being more or less equally involved. At the present time, these fractures appear to be increasing, probably due to the increase in road traffic and the fact that the introduction of safety devices, such as the crash helmet and safety belt, preserve the life of many people would otherwise have been killed at the time of the accident. Emerging treatments are:

• 1. maintain the airway,
• 2. control hemorrhage,
• 3. treatment of fracture.

  The production of monoclonal antibodies against different keratin proteins makes it possible to study keratin expression in tissue under various experimental conditions. The objectives of this study are to examine bone regeneration using routine histologic technique and to investigate the change in the cytokeratin expression of healing keratinocytes in dog tooth extractions wound using immunohistochemical technique.

Three matured mongrel dogs were used; thirty-six premolar teeth were extracted and the healing process examined at 5, 14 and 30 days.

For routine histologic examination, the tissue were cut at 5 microns and stained with hematoxylin and eosin.

For immunohistochemical evaluation, the tissue were fixed in methacarn, dehydrated in absolute alcohol, cleared in xylene, infiltrated and embedded in paraffin. Five micron sections were cut and stained Avidin/Biotin immuno-peroxidase technique employing AE₈, AE_]4 and PKK₂ antibodies.

Five-day old sockets showed osteoblastic and osteoclastic activity. Thirty-day old sockets were filled with immature trabecular bone. Epithelial proliferation was noted in five-day wounds and was apparently complete by 14 days. There was change in the cytokeratin expression of healing keratinocytes as indicated by PKK₂ localization at basal and suprabasal cell layers in 5, 14 and 30 day wounds.

In conclusion, the proliferating keratinocytes showed an increased keratin expression, typical of proliferative cells.
Saudi Dental Journal 1992;4(SI)-Abstr.09:p10.

Damage to the TMJ may be traumatic, inflammatory, metabolic, necrotic or iatrogenic. The purpose of this clinical evaluation was to establish the protocol for the usage of a new Continuous Passive Motion (CPM) machine and to revierw the relevant literature. Continuous motion of injured or surgically treated joints has been advocated since 1877. There have been two schools of thought in Orthopedics: The Immobilizers and the Mobilizers. Recent research, both animal and clinical studies have demonstrated the effectiveness of CPM.