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| 2010-22 |
| 22-1 |
ISSN (Print) 1013-9052
EISSN 1658-3558
The Saudi Dental Journal,
P.O. Box 52500,
Riyadh 11563,
Kingdom of Saudi Arabia
P.O. Box 52500,
Riyadh 11563,
Kingdom of Saudi Arabia
| Tel. |
966-1-467-7328 |
| Fax. |
933-1-467-7308 / 966-1-467-7534 |
| Email |
saudidj@ksu.edu.sa |
|
The effect of different temperature and concentration of sodium hypochlorite on the elimination of E.Faecalis using rotary instrumentation and intermittent passive ultrasonic irrigation Ebtissam M. Al-Madi, BDS, MSc
Department of Restorative Dental Sciences
College of Dentistry, King Saud University P.O. Box 285072, Riyadh, Saudi
Arabia Email:
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OBJECTIVE: The purpose of
this study was to compare the intracanal bacterial reduction using rotary
instrumentation and intermittent passive ultrasonic irrigation (IPUI) with
different concentrations and temperatures of NaOCl in different canal tapers. MATERIALS
and METHODS: The root canals of seventy-two extracted single-rooted
teeth were instrumented up to size #20k file and inoculated with E.
faecalis. The teeth were divided into 5 experimental groups and one
control. The root canals in the control group were shaped to a 0.04 taper using
ProFile rotary files, with 1.5 minute of IPUI by NaOCl at a concentration of
2.5% and room temperature of 25°C for 30 seconds at a time at three intervals.
In Group I, the canals were shaped to a 0.06 taper, and in Groups II and III -
the temperature of NaOCl used was 37°C and 45°C respectively, and in Groups IV
and V - the concentrations of NaOCl were 1% and 5% respectively. The canals
were incubated at 37°C for 48 hours and bacterial samples were obtained using
paper points and plated on agar plates. The zones of bacterial growth were
measured and statistical analysis was performed. RESULTS: There
was significantly more bacterial growth in the control group than in Groups I,
II, III and V. Furthermore, there was a significant reduction in bacterial
growth in Group V compared to Group IV. CONCLUSIONS: The result
of this study showed that significant bacterial reduction in contaminated root
canals could be obtained using intermittent passive ultrasonic irrigation
combined with 2.5% NaOCl at 37°C in canals prepared to a taper of 0.06. In
addition, complete bacterial eradication could be obtained using IPUI with 2.5%
NaOCl at 45°C or 5% NaOCl at room temperature (37°C).
Saudi Dental Journal 2008;20(3):150-155. |